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Quality filtering of the lengthy soar sequences (20-, 40-, and 100-kb insert sizes) was carried out by the suppliers. We have presently analyzed sequences made up of SSR motifs in the genome and determined additional than 22,000 candidates that can be applied to layout primers (Additional file 1: Table S19). To accelerate marker-assisted breeding of yam, we executed genome analysis of white Guinea yam (Dioscorea rotundata) and assembled a 594-Mb genome, 76.4% of which was dispersed among the 21 linkage groups. These genomic sources broaden our information of Guinea yam genetics and present a system for implementing genomics-assisted breeding by marker-assisted range (MAS) in this crucial staple crop. Molecular markers, such as uncomplicated sequence repeats (SSRs), indels, and SNPs, can, for the very first time, be made for a variety of applications in Guinea yam, which include linkage mapping, genome-broad association evaluation, genomic collection, and MAS. Development of DNA markers joined to agronomically essential characteristics and their use for MAS boost the job yam performs in guaranteeing food stability for resource-inadequate households in Africa and beyond. Self-pollination amongst male and female bouquets of ZW monoecious crops could come to be doable, which may perhaps let inbred lines to be produced, letting fixation of wanted alleles of agronomically important features. The skinny blue traces shown less than the initial, 2nd, and third horizontal strains indicate the positions of feminine contigs (P3-DDN) exclusively mapped by F1 woman bulk reads